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Apr 2018 DOI 10.14302/issn.2690-4829.jen-18-2025
Phillip BrummCorresponding author
The goal of this work was to clone, express, characterize and assemble a set of soluble thermostablecellulases capable of significantly degrading cellulose. We successfully cloned, expressed, and purified eleven Clostridium thermocellum (Cthe) cellulases and eight Acidothermuscellulolyticus(Acel) ce…
Dec 2019 DOI 10.14302/issn.2690-4829.jen-19-3105
Phillip BrummCorresponding author
Trichoderma reeseiβ-glucosidase (Bgl1) is one of four enzymes demonstrated to act synergistically to degrade cellulose both in vitro and in vivo. Our work attempted to better understand the substrate specificity and potential biotechnological applications of Bgl1. T. reesei Bgl1H cleaves over 80% of…
Mar 2019 DOI 10.14302/issn.2471-2140.jaa-19-2699
Hassan M.M. MasoudCorresponding author
Xanthine oxidase is a commercially important enzyme with wide area of medical applications to develop diagnostic kits. Xanthine oxidase was extracted, purified and characterized from sheep liver (SLXO). The purification procedure involved acetone precipitation and chromatography on DEAE-cellulose an…
Dec 2018 DOI 10.14302/issn.2694-2275.jzr-18-2474
Emile MiégouéCorresponding author
Guinea pig diet is essentially based on the use of grasses associated with protein sources. Then, in to improve the herbivores nutrition, the evaluation of intake and In vivo digestibility of Pennisetum purpureum or Panicum maximum in guinea pigs (Cavia porcellus) was carried out in January 2017 at…
Aug 2018 DOI 10.14302/issn.2639-3166.jar-18-2264
Giorgio MasoeroCorresponding author
The management of symbiotic Microbial Biota (MB) in the soil as agents that promote the yield and health of crops, is aimed at inducing modifications of the phenotype of plants, both over and under the ground. It is here shown, in Sorghumsudanensis plants, that: i) a simple response to MB inoculatio…
Feb 2014 DOI 10.14302/issn.2328-0182.japst-13-206
J. C. García-RodríguezCorresponding author
The purpose of this study was to investigate the effect of cremophor RH-40 and polysorbate 80 with hydroxypropyl methylcellulose (HPMC) F4M on the development of formulations of intranasal erythropoietin with low sialic acid content (Neuro-EPO) as a neuroprotective agent. Parameters such as pH, osmo…