Search results for “PCR”

About 44 results in articles

Open Access Pub publishes peer-reviewed, free-to-read open-access articles. Showing articles matching PCR — open any to read the full text, or download the PDF or XML.

Comparative Analysis of Five Commercial RT-PCR Diagnostic Assay for Detection of Covid-19

Sep 2023 DOI 10.14302/issn.2692-1537.ijcv-23-4660
Saeid Amel JamehdarCorresponding author

SARS-CoV-2 real-time reverse-transcription PCR (rRT-PCR) is the most effective testing system available to combat COVID-19, given the absence of any specific treatment or vaccine. Moreover, numerous SARS-CoV-2 rRT-PCR kits have been approved under emergency-use-authorization (EUA) worldwide. In this…

Quantification of Micrornas by Absolute Dpcr for the Diagnostic Screening of Colon Cancer

Feb 2019 DOI 10.14302/issn.2471-7061.jcrc-18-2526
Farid E. AhmedCorresponding author

There is currently no validated micro(mi)RNA diagnostic stool test to screen for colon cancer (CC) on the market because of the complexity of fecal density, vulnerability of stool to daily changes, and the presence of three sources of miRNAs in stool (cell-free from fecal homogenates, exsosomal miRN…

The Development and Evaluation of A Multiplex Real-Time PCR Assay for the Detection of ESBL Genes in Urinary Tract Infections

Aug 2018 DOI 10.14302/issn.2690-4721.ijcm-18-2217
Shivanthi SamarasCorresponding author

Background Overuse of beta-lactam antibiotics has lead to selection for extended-spectrum β-lactamase (ESBL) producing Enterobacteriaceae, a major cause of antibiotic resistant urinary tract infections (UTIs). Standard detection methods are time-consuming, with disputed accuracy. This study describe…

Proteomic and Genomic Techniques in Medical Research: Applications in Cancer, Diagnostics, and Personalized Medicine

Nov 2025 DOI 10.14302/issn.2326-0793.jpgr-25-5573
Oghenetega E. ImiruayeCorresponding author

Advancements in proteomic and genomic technologies have transformed molecular biology by enabling comprehensive analysis of biological systems at the molecular level. This literature review explores the evolution, methodologies, and practical applications of key proteomic and genomic techniques. In…

Cervical Cancer with The Active And Stable PI3K/MTOR/AKT Pathway In Azerbaijan Patients

Mar 2024 DOI 10.14302/issn.2997-2108.jcc-23-4838
Konul S. MardanovaCorresponding author

Among the reproductive cancers cervical cancer has special place, because the second most frequent cause of cancer-related death among women worldwide. The studies suggested that the PI3K/mTOR/AKT signaling pathway is associated with certain reproductive tumors. A lot of research is ongoing for unde…

Human Immunodeficiency Virus Drug Resistance (HIVDR) and Baseline Characteristics among Antiretroviral Therapy (ART) experienced Children and Adolescents under the care of Chidamoyo Christian Hospital in Hurungwe, Zimbabwe

Dec 2023 DOI 10.14302/issn.2324-7339.jcrhap-23-4634
Alfred MakuraCorresponding author

Introduction Human Immunodeficiency Virus (HIV) remains a persistent global public health challenge. In 2020, approximately 37.9 million individuals were living with HIV globally, including 1.7 million children <15 years old, with a global HIV prevalence of 0.8% among adults. A larger portion of peo…

Camel Brucellosis in Ethiopia: Seroprevalence and Associated Risk Factor

Oct 2023 DOI 10.14302/issn.2575-1212.jvhc-23-4532
Sultan Abda NejaCorresponding author

Camels are a significant source of income for nomadic populations in many developing countries, including Ethiopia. Camels are well adapted to dry and semi-dry regions, providing income, food security, and transportation. However, camel production and productivity are constrained by infectious disea…

A Review of Attempts to Identification and Antifungal Susceptibility of Dermatophytes (Microsporum Canis and Tricophyton Mentagrophytes) Isolated from Infected Cats and Dogs with Experimental Dermatophytosis of Guinea Pigs

Jul 2023 DOI 10.14302/issn.2575-1212.jvhc-23-4510
Sohir YoussefCorresponding author

Dermatophytosis affect companion animal’s skin and keratin appendages as cats and dogs, resulting in red, scaly, itchy, bald, and raised patches like ring. The three main groups are Microsporum, Trichophyton and Epidermophyton. This study collected samples of skin scrapping and hairs from 130 cats a…

Research Gaps in Contaminants of Emerging Concern (CECs): Routes to the Standardization of Chemical Test Methods By GC/LC-Mass Spectrometry: A Review

Jun 2023 DOI 10.14302/issn.2377-2549.jndc-23-4615
Thavrin ManickumCorresponding author

A literature review was undertaken with a focus on 1) identifying the research gaps regarding CECs, 2) identifying the most common ones, and 3) identifying the typical analytical methods/technologies employed, for their analysis. A total of 214 papers were noted, with a total of 21 review articles (…

Detection of carbapenem resistance mechanisms among Avian Pathogenic Escherichia coli (APEC) isolated from broiler chickens

May 2023 DOI 10.14302/issn.2575-1212.jvhc-23-4521
Aya El- shaerCorresponding author

Background The emergence and spread of carbapenem-resistant gram-negative bacteria pose a serious threat to human health. Currently, little is known about the molecular mechanisms underlying carbapenem -resistance and their prevalence among APEC in Egypt. The aim of this study was to detect APEC in…

Detection of Smuggled Genetically Modified Crops and Assessment of its Environmental Impact in the Ethio -Sudan Trans Boundary Area, North West Ethiopia

Apr 2023 DOI 10.14302/issn.2576-6694.jbbs-22-4390
Nega BerhaneCorresponding author

Background The genetic material of the genetically modified crop has been altered to develop the necessary insect resistance features by introducing genes from the Bt (Bacillus thuringiensis) bacterium. The objective of this study was to find smuggled GM Bt crops in the Metema farming area and exami…

The Impact of Chest Computed Tomography in A Covid-19 Reference Hospital - First Wave - Distrito Federal - Brazil

Sep 2022 DOI 10.14302/issn.2692-1537.ijcv-22-4328
Gleim Dias De SouzaCorresponding author

Objectives To evaluate the diagnostic accuracy of chest CT for the diagnosis of COVID-19 associated with the clinical presentation and in relation to the PCR-RT. Sensitivity, specificity, positive predictive value and negative predictive value, gender, age group and degree of lung involvement will b…

Molecular Detection of Mycoplasma Gallisepticum and Mycoplasma Synoviae Infection in Poultry

Aug 2022 DOI 10.14302/issn.2835-2165.jfsh-22-4263
Waqar MazharCorresponding author

In Pakistan, production of poultry has been evolved as a good alternate of beef and mutton. In this study multiplex PCR approaches were adopted to differentiate and detect avian mycoplasma in a single PCR reaction as a step forward in the economization of PCR detection. From the total of 25 samples,…

Targeting Mutational Landscape of TP53 in patients diagnosed with Oral Cancer living in Senegal

Mar 2022 DOI 10.14302/issn.2572-3030.jcgb-22-4121
Pierre Diaga SARRCorresponding author Laboratory of Clinical Cytology, Cytogenetics and Reproduction Biology, Aristide Le Dantec Hospital, Dakar-Senegal

Introduction Genomic mutations in TP53 gene in association with etiological risk factors have been associated with oral carcinogenesis. Herein, we screened for genomic variants of TP53 predisposing to oral cancers in Senegalese patients. Methodology 88 patients with confirmed diagnostic were recruit…

Validity of PIRO Score as an Assessment Tool for Mortality Risk of COVID-19 Pneumonia among Patients Admitted to World Citi Medical Center from March to August 2020. A Retrospective Observational Cohort Study

Dec 2021 DOI 10.14302/issn.2692-1537.ijcv-21-4045
Graciano D. Natividad IIICorresponding author

Introduction In December 2019, cases of serious illness causing pneumonia and death were first reported in Wuhan, China.2 The clinical features of Corona Virus Disease-19 (COVID-19) are ranging from asymptomatic to multi organ dysfunction. The disease can progress to pneumonia, respiratory failure a…

Cytokine Expression in Peripheral Blood Mononuclear Cell Cultures Obtained from Cattle with Different Stages of Natural Mycobacterium bovis Infection

Dec 2021 DOI 10.14302/issn.2575-1212.jvhc-21-4034
Fernando Díaz-Otero,Corresponding author CENID-Salud Animal e Inocuidad. Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP). Carretera México-Toluca, Km. 15.5, C.P. 05110, Ciudad de México, México.

In bovine tuberculosis (bTB), cellular, humoral, or both types of immune responses have been observed. The purpose of this study was to examine the immune status of tuberculous cows based on the differential cytokine gene expression associated with Th1 (IFN-γ, IL-2), or Th2 (IL-4, IL-10) responses.…

SARS-Cov-2 Viral Kinetics in Mild COVID-19 Patients Treated with Chloroquine Regimens or Standard of Care

Aug 2021 DOI 10.14302/issn.2692-1537.ijcv-21-3924
Krittaecho SiripassornCorresponding author

This study measures the impact of chloroquine (CQ) therapy in reducing SARS-CoV-2 viral load in infected individuals and hence its transmissibility by describing changes in nasopharyngeal SARS-CoV-2 RNA kinetics in patients receiving standard of care (SOC) or CQ +/- ritonavir-boosted lopinavir (LPV/…

The Evolution of the Enzyme Immunoassay/Enzyme-Linked Immunosorbent Assay

Aug 2021 DOI 10.14302/issn.2326-0793.jpgr-21-3917
Leonid TarassishinCorresponding author

50 years ago the Enzyme Immunoassay Enzyme-Linked Immunosorbent Assay, mostly known as ELISA was developed. This is a powerful but simple method that is very widely used in the diagnostic practice, as well as in biomedical research. During this time a number of ELISA modification were developed that…

Use of the Remaining Sample from the Panbio COVID-19 Antigen Rapid Test Device for the Molecular Screening of the SARS-CoV-2 Variant of Concern B.1.1.7

May 2021 DOI 10.14302/issn.2690-4721.ijcm-21-3835
Milagrosa MontesCorresponding author

Objective Real-time surveillance of SARS-CoV-2 variants of concern (VOC) is of essential public health importance. Rapid Antigen Detection Tests (RAgDT) have become first-line COVID-19 diagnostic methods in many regions, but this strategy can hamper the surveillance of the virus variants due to thei…

Lennert Lymphoma (lymphoepithelioid lymphoma) with Extensive Necrosis: A Case Report

Apr 2021 DOI 10.14302/issn.2372-6601.jhor-21-3801
Xin QingCorresponding author

Lennert lymphoma (lymphoepitheloid lymphoma) is an extremely rare variant of peripheral T-cell lymphoma, not otherwise specified. Here we report a case of Lennert lymphoma diagnosed in a 57-year-old woman. She had a three-year history of waxing and waning lymphadenopathy with a rapid increase in siz…

Frequently asked questions

Are these articles peer-reviewed?
Yes. Articles published at Open Access Pub go through single-blind peer review (double-blind on request) under an editorial board before publication.
Are the articles free to read?
Yes. Every article is open access — read the full text online for free and download the PDF or XML, with no paywall or subscription.
How do I cite an article?
Use the DOI shown on each result and on the article page; it is the permanent, citable link to the article.
How do I read or download an article?
Click "Read full text" to open the article HTML, or use the PDF / XML buttons on each card to download it.