Journal of Proteomics and Genomics Research
Open Access
Optimization and ZSPORE Analysis of Affinity Purification Coupled with Tandem Mass Spectrometry in Mammalian Cells
Dec 2012 DOI 10.14302/issn.2326-0793.jpgr-12-100
Shitao LiCorresponding author
Defining protein-protein interactions is essential for understanding the mechanisms by which cells regulate basic functions, such as metabolism, transcription, and signal transduction. Affinity purification followed by tandem mass spectrometry (AP-MS) has application for discovery of new interactors…